Gardnerella vaginalis

  • gram variable / gram (-)
  • beta-hemolytic (HBT agar) / nonhemolytic (BAP)
  • causes bacterial vaginosis

Causes Bacterial Vaginosis 

  • Gardnerella vaginalis 
  • Prevotella spp. 
  • Peptostreptococcus spp. 
  • Porphyromonas spp. 
  • Mobiluncus spp. 
  • Mycoplasma hominis

NUGENT SCORING SYSTEMmost accurate means of diagnosing bacterial
vaginosis than culture

NUGENT SCORING SYSTEM

Lactobacillus morphotypes (boxy, gram (+) bacilli

Gardnerella & Bacteroides
(pleomorphic, gram variable, gram (-), short bacilli)
Mobiluncus morphotypes (curved, gram-variable bacilli)
Quantity Points Quantity Points Quantity Points
4+ 0 0 0 0 0
3+ 1 1+ 1 1+ to 2+ 1
2+ 2 2+ 2 3+ to 4+ 2
1+ 3 3+ 3  
0 4 4+ 4  

NUGENT SCORING INTERPRETATION

  • 0 – 3 – NORMAL VAGINAL MICROBIOTA
  • 4 – 6 – INDETERMINATE FOR BACTERIAL VAGINOSIS
  • 7 – 10 – BACTERIAL VAGINOSIS