Liver Function Test

METHODS

• Van den Bergh: color reaction for bilirubin
  • Color reagent: Diazo reagent
  • Product: Azobilirubin
  • Evelyn-Malloy
    • Medium: ACID
    • Dissociating agent: 50% methanol
    • End color: red/reddish purple
  • Jendrassik-Grof
    • Medium: ALKALINE
    • Dissociating agent: Caffeine sodium benzoate
    • End color: blue
  • Icterus index
    • Applicable to newborn and neonates
  • Bromsulfonpthalein Dye Excretion test
    • Rosenthal White
      • Double collection method
      • Collection is done after 5 mins and 30 mins
      • Reference values: 50% dye retention (5mins) 0% (30mins)
    • Mac Donald
      • Single collection method
      • Collection: done after 45 mins (+ 5% dye retention)

DISEASES

• Gilbert syndrome: defect in transport protein in liver
• Crigler-Najjar syndrome: defective conjugation due to deficiency of UDP-GTase
• Dubin-Johnson syndrome: defective excretion due to blockage by stones