Haemophilus

  • Gram (-) coccobacilli 
  • Pleomorphic 
  • Requires X and V factor 
  • Facultative anaerobes
 XVPorphyrinOthers
H. haemolyticus + + Beta-hemolysis in Horse BAP
H. aegypticus
(Koch-Weeks
Bacillus)
+ + Pink-eye conjunctivitis; Brazilian Purpuric fever
H. influenzae
(Pfeiffer’s bacillus)
+ +

Virulence factor: Type B capsule, IgA protease, pili, LPS – has paralyzing effect on ciliated respiratory epithelium.

Major cause of epiglottitis Otitis media, pneumonia, cellulitis.

H. parainfluenzae + + Primary site of infection – mitral valve
H.
parahaemolyticus
+ + Beta-hemolysis on Horse BAP
H. paraphrophilus + + 
H. ducreyi + SOFT CHANCRE, school of fish, grows well @ 33°C
H. aphrophilus + 

SPECIMEN COLLECTION 

  • Haemophilus spp. are susceptible to DRYING and TEMP. EXTREMES. 
  • Lower RT spx: bronchoalveolar lavage 
  • Pneumonia and CSF infection – STERILE FLUID AND BLOOD 
  • H. ducreyi genital ulcers