Suggested Guidelines for Record and Specimen Retention Record / Specimen Type Retention Requisition 2 years Accession Logs 2 years Maintenance / Instrument logs 2 years Quality Control Records 2 years Blood bank donor / receipt records 10 years Blood bank patient records 10 years Blood bank employee signatures / initials 10 years Clinical Pathology test records …
Fixation: 50% alcohol – all types 70% alcohol – sputum 95% alcohol – most commonly used; urine, bronchial & gastric Equal parts of ethanol & ether → BEST fixative! (but highly volatile & flammable) Saccomano’s fixative → 50% ethanol + 2% carbowax Spray fixatives – 1 ft. away! 2-3 slides should be prepared per patient …
ABNORMALITIES IN CELL GROWTH RETROGRESSIVE CHANGES Agenesis Complete non-appearance of an organ Aplasia Incomplete or defective development (usually seen inpaired organs) Atresia Failure of an organ to form an opening Atrophy Partial or complete decrease in size of normally mature organ Hypoplasia Failure of an organ to reach its matured size PROGRESSIVE CHANGES Hyperplasia Increased …
FRESH TISSUE EXAMINATION No addition of chemicals (eg. Fixatives) 1. Teasing – tissue is placed in a watch glass with isotonic solution then observed under a microscope 2. Squash Preparation (Crushing) – <1mm tissue is literally crushed (between 2 slides) 3. Smear Preparation Streaking (zigzag pattern) Spreading Pull-apart Touch preparation 4. Frozen Section Uses either …
Histology is the study of tissues Type Location EPITHELIAL TISSUES Simple squamous Endothelium, mesothelium, alveoli,glomerulus Simple columnar Fallopian tube (ciliated), gallbladder(non-ciliated) Simple cuboidal Walls of thyroid follicles Stratifiedsquamous Skin (keratinized), vagina (nonkertinized) Stratified columnar Urethra of male Pseudostratifiedcolumnar Trachea (ciliated) Stratified cuboidal Ducts of sebaceous glands Transitional Linings of urinary tract CONNECTIVE TISSUES …
