Transfusion Reaction Information Prevention / Treatment FNHTR 1°C increase in temperature Cause: Anti-leukocyte antibodies (patient) Leukoreduced RBCs Allergic Cause: donor plasma with foreign proteins Washed RBCs Anaphylactic Patient has IgA deficiency w/ anti IgA antibodies Patient is afebrile (no fever) Washed RBCs IgA-deficient donor (rare) Anaphylactoid Patient has normal IgA with anti-IgA antibodies Patient is …
DIRECT ANTIGLOBULIN TEST Detection of sensitization of red cells in vivo Examples: AIHA, HDN, HTR INDIRECT ANTIGLOBULIN TEST Detection of sensitization of red cells in vitro Sensitization occurred during incubation period Examples: Weak D testing, AHG-phase compatibility testing, antibody screen and identification
Aka serologic crossmatch test Test to detect unexpected antibodies Ensures to produce a blood product safe for transfusion MAJOR CROSSMATCH (DCPS) Tests for alloantibodies in patient’s plasma against donor cells Donor’s cell + Patient’s plasma MINOR CROSSMATCH (DSPC) Tests for alloantibodies in donor’s plasma against patient’s cells POTENTIATORS aka enhancement media (increases reactivity of …
1. Registration collection of patient information date of last donation 2 days or more – after plasmapheresis, plateletpheresis or leukapheresis 4 weeks – after infrequent plasmapheresis 8 weeks – between whole blood donations 16 weeks – after 2-unit red cell collection 2. Health history interview Deferrals Permanent Tegison medication – for severe psoriasis Persons with …
ABO ISBT No. 001 Causes the most severe HTRs O > A > B > A1B > A2B (most common to least common blood type) Antigens are detectable 5-6 weeks in utero Full expression of antigens – 2 to 4 yrs of age Antibodies are naturally occurring Begins to rise at 3-6 mos and peaks …
BLOOD AND BLOOD COMPONENTS WHOLE BLOOD Hct approximately 40% Storage and Shelf life 1-6°C ACD, CPD or CP2D – 21 days CPDA-1 – 35 days PACKED RBCs Hct < 80% Storage and Shelf life 1-6°C ACD, CPD or CP2D – 21 days CPDA-1 – 35 days Open system – 24 hrs RANDOM DONOR PLATELETS (RDP) …
PRESERVATIVE SOLUTIONS Anticoagulant/Preservatives Storage Time Heparin 2 days Acid-Citrate-Dextrose 21 days Citrate-Phosphate-Dextrose Citrate-Phosphate-Double-Dextrose(CP2D) 21 days Citrate-Phosphate-Dextrose-Adenine(CPDA-1) 35 days CPDA-2 42 days AS-1, As-3 and AS-5
YEAR PERSONS INVOLVED RESEARCH/EVENT 1492 Pope Innocent and3 donors First blood transfusion recorded in history 1500 Chinese Variolation; Insufflation– material was dried into a powder andinhaled by non-immune person 1700 Edward Jenner Cross-immunity (exposure to cowpox andimmunity to small pox) 1880 Louis Pasteur Attenuation*, rabies vaccine– bacteria/viruses are weakened 1888 Elie Metchnikoff Phagocytosis 1901 Emil …
