SOUTHERN BLOT Description Specimen Requirements Procedure Interpretation Description This procedure allows the analysis of any gene or gene region in the genome at the molecular level. In 1975, Edward Southern, who first described this method, explained this technique whereby chromosomal DNA could be an agarose gel electrophoresis. Southern blotting can be used to identify microorganisms, …
SEMEN ANALYSIS Description Specimen Requirements Procedure Interpretation Description Semen analysis is the most common diagnostic test in assessing infertility. However, abnormal semen analysismay require additional testing such as sperm viability and morphology. Routin semen analysis includes the evaluation ofthe semen volume, sperm count and concentration, pH, appearance, viscosity, sperm motility. Normal pH volume is 2-5mL. …
SCOTCH TAPE METHOD Description Specimen Requirements Procedure Interpretation Description Enterobius vermicularis or human pinworm, typically characterized by a perianal itching or pruritus ani, has a high prevalence rate not only in third world countries but also in developed countries. To diagnose and confirm such, scotch tape method or cellulose tape method or Graham’s scotch adhesive …
SALT TOLERANCE TEST Description Specimen Requirements Procedure Interpretation Description This is used to differentiate gram (+) cocci that grow in 6.5% NaCl such as Enterococcus and Aerococcus. Specimen Requirements Isolated colonies on sheep blood agar. Procedure • Medium: 6.5 % NaCl broth1. Using a sterile loop pick one or two isolate colonies from …
RADIOIMMUNOASSAY (RIA) Description Specimen Requirements Procedure Interpretation Description RIA is the first immunoassay being developed and pioneered by Yalow and Berson in 1969. Following the principle of half-life, the most popular radioisotope label being used used is 125I with a half-life of 60 days. RIA is well known as a sensitive in vitro assay technique …
RF LATEX TEST Description Specimen Requirements Procedure Interpretation Description Rheumatoid factor is used as a diagnostic test for rheumatoid arthritis, an inflammatory condition of the joints. Itis considered as an IgM type of immunoglobulin directed against the Fc region of IgG. A titer of 1:80 is believed to be normal. The RF latex test follows …
REVERSE CAMP TEST Description Specimen Requirements Procedure Interpretation Description Reverse CAMP test is now used as a tool for the presumptive identification of Clostridium perfringens which is reverse CAMP positive. The CAMP factor produced by Streptococcus agalactiae Is used for identifying C. perfringens from other Clostridium species. To be specific, the alpha toxin produced by …
RETICULOCYTE COUNT Description Specimen Requirements Procedure Interpretation Description A reticulocyte is an immature erythrocyte with remnants of aggregates of RNA. The reticulocyte count with its associated corrections, is used to assess bone marrow erythropoietic activity. Specimen Requirements Whole blood anticoagulated with EDTA is recommended. Capillary blood is also acceptable Procedure 1. Mix equal amounts of …
QUELLUNG REACTION Description Specimen Requirements Procedure Interpretation Description Quellung reaction or Neufeld-Quellung test or capsular swelling test is the gold standard technique for detecting the serotypes of Streptococcus pneumoniae. The test involves antigen antibody reactions microscopically using specific antisera to pneumococcal cell suspension. Anticapsular antibodies present in the serum react with the carbohydratematerial on the …
QBC TEST Description Specimen Requirements Procedure Interpretation Description Unlike the conventional Giemsa thin and thick blood smears for malarial detection, the quantitative buffy coat test provides rapid malarial detection with a greater level of sensitivity. This test is considered simple and less time consuming because of the necessary staining reagents already contained within a single …
