Microbiology

Protozoans

E. histolytica Transmission Fecal-oral Morphology Oocyst  Troph: bullseye shaped nucleus; RBC in cytoplasm Clinical Findings Asymptomatic carrier Bloody diarrhea Liver abscess Diagnosis Fecal smear Serology CT Scan G. lamblia Transmission Fecal-oral Morphology Oocyst  Flagellated trophozoite Clinical Findings Foul smelling,greasy diarrhea Diagnosis Fecal smear Immunoassay Isospora spp. Transmission Fecal-oral Morphology Oocyst Clinical Findings Severe diarrhea and …

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Trematodes

Known as flukes  Requires 2 intermediate hosts exc. Schistosomes  1st – snail  2nd : FISH H. heterophyes,C. sinensisO. felineus CRAB P. westermani PLANT F. hepaticaF. giganticaF. buski SNAIL E. ilocanum BLOOD FLUKES  S. japonicum – small lateral knob; oriental blood fluke; katayama syndrome; swimmer’s itch; snail fever  S. mansoni – prominent lateral; smallest blood …

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Cestodes

Tapeworm; flat and ribbon-like  Hermaphrodite; lack digestive organs Body  Scolex (head)  Neck (region of growth)  Proglottids (strobila) Taenia spp   Taenia solium Taenia saginata Common name Pork Tapeworm Beef tapeworm Intermediate host Pig; man Cattle Scolex w/ rostellum armedwith 2 rows of large &small hooklets No rostellar hooks4 prominent acetabula Length <7 meters <25 meters …

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Blood & Tissue Nematodes

SUBCUTANEOUS  Loa loa  Mansonella streptocerca  Onchocerca volvulus SEROUS CAVITY  Mansonella spp. LYMPHATIC  Wuchereria bancrofti  Brugia malayi   W. bancrofti B. malayi Mean length (um) 290 222 Cephalic space:breadth 1:1 2:1 Sheath in giemsa Unstained Pink Nuclei Regularly spaced;separate Irregularly spaced;overlapping Tail Single row of nuclei;does not reach tail end Single row of nucleireaches the tail …

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Nematodes

Characteristics  Cylindrical, elongated, & bilaterally symmetrical  Anterior end equipped with hook, teeth, plates and papillae  Alimentary tract is simple, extending from mouth to anus. NO CIRCULATORY SYSTEM UNHOLY THREE Hookworm Ascaris lumbricoides Trichuris trichiura HEART-LUNG MIGRATION Ascaris lumbricoides Strongyloides stercoralis Hookworm SMALL INTESTINE Capillaria philippinensis Hookworm Ascaris lumbricoides Trichuris trichiura Strongyloides stercoralis LARGE INTESTINE Enterobius …

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Mycobacteria

Slender, rod-shaped (0.2- 0.6 um x 1- 10um in size)  Nonmotile; non-spore formers  Strictly aerobic  Increased CO2 – enhances growth RAPID GROWERS  Grows in simple media  Grows 2-3 days  20-40°C DISEASE-ASSOCIATED MYCOBACTERIA  Requires 2-6 weeks  Requires complex media  Has specific optimal temp. MTB COMPLEX  M. microti – TB in immunocompetent and compromised  M. africanum – …

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Anaerobic Organism

Gram positive SPORE-FORMING BACILLI Clostridium perfringens Clostridium botulinum Clostridium tetani Clostridium difficile Clostridium septicum Gram positive Bacilli Actinomyces spp. Propionibacterium spp. Bifidobacterium spp. Gram negative bacilli Bacteroides fragilis Porphyromonas spp. Prevotella spp. Fusobacterium spp. Gram negative cocci Veillonella spp Clostridium perfringens Virulence Factor Alpha & Beta toxins  Type A (mild)  Type C -food poisoning (enteritis …

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Neisseria And Moraxella

Gram (-) cocci Positive for Catalase Oxidase Superoxol (Neisseria) Glucose fermenters exc. For Moraxella (asaccharolytic) Gonorrhea Flow of seeds “Clap” – “clapoir” (French) – brothel Disease Association N. gonorrhoeae Leading cause of sexually transmitted disease always pathogenic; not a normal flora Opthlamia neonatorum N. meningitidis Leading cause of fatal bacterial meningitis Waterhouse friderichsen syndrome Endemic …

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Streptobacillus And Spirillum

  Streptobacillusmoniliformis Spirillum minus General characteristics 1. Requires blood, serum or ascite fluid in the mediumand incubation under CO22. Facultative, nonmotile anaerobe3. Highly pleomorphic Gram (-), helical, strictlyaerobic Disease Association Haverhill fever Ratbite fever (SODOKU) Lab diagnosis Blood Blood, exudate, orlymph node tissues Direct Detections Pus or exudates – stained with gram or giemsa stain …

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Francisella

Gram (-) coccobacilli  Strict aerobes  Urease (+) motility (-) oxidase (-)  MAJOR VIRULENCE FACTOR – CAPSULE Francisella spp. Disease association Tularemia – one of the most common lab acquired infection – Rabbit fever  Deer fly fever  Market men’s disease Laboratory diagnosis BSL Level 2 Pathogen Specimen: scrapings from infected ulcers  lymph node biopsies  sputum Whole …

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