AMERICAN TRUDEAU SOCIETY MEDIUM Egg-based Purpose – isolation of M. tuberculosis Component: Eggs – provides fatty acid Potatoes – carbon source Malachite green – inhibitor LOWENSTEIN-JENSEN MEDIUM Purpose – for cultivation of Mycobacterium spp. Component: Potato flour Egg Glycerol Asparagine – for max. production of niacin by certain Mycobacterium spp. Malachite green – inhibitor MODIFICATIONS …
ACETATE AGAR Purpose differentiate E. coli from Shigella spp. Components Acetate – carbon source Bromthymol blue – pH indicator RESULT Green – negative (didn’t utilized acetate) Blue – positive (utilized acetate) ALKALINE PEPTONE WATER Purpose for recovery of Vibro spp. And Aeromonas spp. Components 0.5 – 1.0% NaCl – to recover Vibrio spp. BACTEROIDES BILE …
COLLECTION STOOL COLLECTION (TYPICAL) one spx. collected every other day total of three collected in 10 DAYS Diagnosis of Amoebiasis six specimens in 14 DAYS If patient in therapy of BISMUTH, BARIUM, & MINERAL OIL collect PRIOR TO THERAPY not until 5-7 or 4 – 5 DAYS after completion of therapy DELAYED FOR 2 WEEKS …
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PRINCIPLES OF SPECIMEN COLLECTION Major goal of microbiology laboratory – aid diagnosis of infectious disease Goal of specimen collector – maintain viability w/ minimal contamination FUNDAMENTALS Collect in acute phase of infection and before antibiotics administration (or within 2-3 days for viral infection) Correct anatomic site for collection Proper technique and supplies w/ minimal contamination …
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