Parasitology

Protozoans

E. histolytica Transmission Fecal-oral Morphology Oocyst  Troph: bullseye shaped nucleus; RBC in cytoplasm Clinical Findings Asymptomatic carrier Bloody diarrhea Liver abscess Diagnosis Fecal smear Serology CT Scan G. lamblia Transmission Fecal-oral Morphology Oocyst  Flagellated trophozoite Clinical Findings Foul smelling,greasy diarrhea Diagnosis Fecal smear Immunoassay Isospora spp. Transmission Fecal-oral Morphology Oocyst Clinical Findings Severe diarrhea and …

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Trematodes

Known as flukes  Requires 2 intermediate hosts exc. Schistosomes  1st – snail  2nd : FISH H. heterophyes,C. sinensisO. felineus CRAB P. westermani PLANT F. hepaticaF. giganticaF. buski SNAIL E. ilocanum BLOOD FLUKES  S. japonicum – small lateral knob; oriental blood fluke; katayama syndrome; swimmer’s itch; snail fever  S. mansoni – prominent lateral; smallest blood …

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Cestodes

Tapeworm; flat and ribbon-like  Hermaphrodite; lack digestive organs Body  Scolex (head)  Neck (region of growth)  Proglottids (strobila) Taenia spp   Taenia solium Taenia saginata Common name Pork Tapeworm Beef tapeworm Intermediate host Pig; man Cattle Scolex w/ rostellum armedwith 2 rows of large &small hooklets No rostellar hooks4 prominent acetabula Length <7 meters <25 meters …

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Blood & Tissue Nematodes

SUBCUTANEOUS  Loa loa  Mansonella streptocerca  Onchocerca volvulus SEROUS CAVITY  Mansonella spp. LYMPHATIC  Wuchereria bancrofti  Brugia malayi   W. bancrofti B. malayi Mean length (um) 290 222 Cephalic space:breadth 1:1 2:1 Sheath in giemsa Unstained Pink Nuclei Regularly spaced;separate Irregularly spaced;overlapping Tail Single row of nuclei;does not reach tail end Single row of nucleireaches the tail …

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Nematodes

Characteristics  Cylindrical, elongated, & bilaterally symmetrical  Anterior end equipped with hook, teeth, plates and papillae  Alimentary tract is simple, extending from mouth to anus. NO CIRCULATORY SYSTEM UNHOLY THREE Hookworm Ascaris lumbricoides Trichuris trichiura HEART-LUNG MIGRATION Ascaris lumbricoides Strongyloides stercoralis Hookworm SMALL INTESTINE Capillaria philippinensis Hookworm Ascaris lumbricoides Trichuris trichiura Strongyloides stercoralis LARGE INTESTINE Enterobius …

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Collection, Transport, Processing, and Staining of Specimens

PRINCIPLES OF SPECIMEN COLLECTION Major goal of microbiology laboratory – aid diagnosis of infectious disease Goal of specimen collector – maintain viability w/ minimal contamination FUNDAMENTALS Collect in acute phase of infection and before antibiotics administration (or within 2-3 days for viral infection) Correct anatomic site for collection Proper technique and supplies w/ minimal contamination …

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