Brucella
- Poorly stained by conventional gram stain
- Resembles fine grain of sand
- Requires erythritol
- Urease (+), catalase (+)
| Brucella | |
| Disease association | Brucellosis-zoonosis, systemic infection |
| Lab Diagnosis | Blood (routine), Bone marrow (preferred), CSF, pleural, synovial, urine, abscesses, other tissues. |
| Media and cultivation | – Brucella agar or infusion base-spx other than blood – 5% heated horse or rabbit serum enhances growth on all media
|
| B. abortus | B.melitensis | B. suis | B. canis | |
| CO2 required for growth | +/- | – | – | – |
| Time to positive in urease | 2 hrs | 2 hrs | 15 mins | 15 mins |
| H2S produced | + | + | – | – |
| Thionine | + | – | – | + |
| Fuchsin | – | – | – | + |
