Campylobacter, Helicobacter
- Gram (-) bacilli
- Microaerophilic (5-10% O2)
| Campylobacter | Helicobacter | |
| Disease Association | Most common cause of gastroenteritis WORLDWIDE 1. Febrile systemic disease 2. Periodontal disease 3. Gastroenteritis Postinfection complication: 1. Reactive arthritis 2. Guillain-Barre Syndrome | H. pylori 1. Can cause peptic ulcer disease & gastric carcinoma, 2. Major cause of Type B gastritis |
| Laboratory Diagnosis | Blood, feces, rectal swabs are acceptable | Tissue biopsies Placed in stuart’s Refrigerated for 24 hrs. |
| Direct Detection | Seagull-winged, S shaped ; DARTING MOTILITY (Hanging drop) | Warthin-Starry or Silver stain and giemsa stain on biopsy specimen. |
| Media and Cultivation | 1. Skirrows 2. Medium V 3. Butzler Medium 4. Campy-CVA 5. Charcoal Cefoperazone Deoxycholate Agar (CCDA) | 1. Brucella agar with 5% SB 2. Selective Media 3. Skirrow’s Media 4. Modified Thayer Martin Agar |
| C. jejuni subsp. jejuni | C. coli | C. fetus | H. pylori | |
| Hippurate hydrolysis | + | – | – | – |
| Growth in 25°C | – | – | -/+ | – |
| Growth @ 42°C | + | + | – | + |
| Catalase | + | + | + | + |
| Urease | – | – | – | + |
| Nitrate to nitrite | + | + | + | +/- |
| H2S in TSI | – | – | – | – |
| Nalidixic acid | S | S | R | R |
| Cephalotin | R | R | S | S |
